#DYNAMIC LIGHT SCATTERING PROTOCOL ISO#

The protocol was robust, accurate and consistent with the ISO standard over the whole range of size that were considered. The present work reports robustness, precision and trueness of this protocol that were investigated using CRM at 100 and 400 nm. In this work, we propose an extension of validation of a protocol of size measurement by DLS previously validated with certified reference materials (CRM) at 60 and 203 nm. However, no protocol of DLS size measurement has been validated over a large range of size so far. This parameter can be explored by dynamic light scattering (DLS) that is described in the ISO standard 22412:2008(E) and is one of the methods recognized by Health Agencies. Thus, Health Agencies have identified the size of nanomaterial as an essential physicochemical property to characterize. If you would like any more information, please do not hesitate to contact us.In vivo fate of nanomaterials is influenced by the particle size among other parameters. This innovative technology has been broadly applied for use in various biopharma applications. We provide dynamic light scattering analyzers suitable for measuring dispersed particles in a liquid as a function of Brownian motion. Meritics is one of the world’s leading suppliers of analytical instrumentation for particle characterization in a broad range of fields. It provides a high-throughput and fully-repeatable measurement methodology for isolated exosome particles in solution, providing confirmation of their particle size and concentration. It has been hypothesized that the membrane vesicle trafficking of enriched exosomes is related to both anti-tumor and pro-tumor activity, implying a functional role in the adaptive immunological responses in the body.ĭynamic light scattering is at the forefront of exosome research and characterization for diagnostic and therapeutic applications. These properties have supported the use of exosomes as novel biomarkers, with additional research into their potential diagnostic applications.

The Importance of ExosomesĮxosomes contain proteins such as mRNA and miRNA which are characteristic of the cells that they were secreted from. It is capable of sizing particles as small as 0.4 nm in sample volumes on the microliter (μl) scale, providing statistically significant data regarding vesicles in solution. The DelsaMax CORE dynamic light scattering particle size analyzer has proven successful in raising the throughput and efficiency of exosome measuring, with the capacity to analyze thousands of exosomes in solution in a single minute. Typically, TEM enables analysis of hundreds of particles over a period of several hours.

These protein-enriched vesicles are believed to play a crucial part in cell-signaling across significant distances within the body.Ĭonventionally, measuring exosomes was challenging for researchers who were forced to establish density gradients by hand and measure the isolated vesicles through time-consuming Transmission Electron Microscopy (TEM). They can be released by almost any cell in the body and are freely available in blood plasma and biological fluids. Dynamic light scattering has emerged as a potential solution to classifying extracellular vesicles of significantly smaller dimensions, enabling scientists to monitor the release of much smaller exosomes from both healthy cells and cells that have come to the end of their lifespan.ĭynamic light scattering is now widely used to characterize isolated exosomes, which are membrane vesicles with cell diameters of approximately 20 – 120 nm. The release of extracellular apoptotic bodies during programmed cell death has long been observed via traditional microscopy, as typical apoptotic mediators display dimensions of >2,000 nm. This has contributed to a general lack of clarity regarding the terminology used to distinguish exosomes, microvesicles, and apoptotic bodies from one another.ĭynamic Light Scattering: Clarifying Exosomes Scientists have been aware of extracellular vesicles for decades, but the infrastructure to precisely characterize distinct vesicle species from one another simply did not exist until recently. These organisms exist on the micron (μm) and submicron (nm) scale and are released by healthy cells into the extracellular environment to facilitate one of many microbiological processes, including coagulation, phagocytosis, and apoptosis. Dynamic Light Scattering for Exosome CharacterisationĮxtracellular vesicles are an overarching species of biological structures that are associated with intercellular communication.

Dynamic light scattering protocol